Facile fabrication and cell imaging applications of. Fabrication of aggregationinduced emission based fluorescent. The discovery of the aie effect opens a new avenue for the. Tang in 2001 to describe situations in which nonemissive or weakly emissive organic molecules exhibit enhanced luminescence when forced into molecular aggregation in the presence of poor solvents or in the solid state. The polymer exhibits unique aggregation induced emission aie characteristics and can selfassemble to sizetunable particles in dmfwater mixtures. Here, we demonstrate this concept by a simple, fast, and robust protocol of cationic polymermediated gold nanocluster au ncs selfassembly into nanoparticles nps of ca. Dear colleagues, aggregation induced emission aie is a term introduced by b. Xiaofan ji, yang li, hu wang, run zhao, guping tang, feihe huang. Tpeqcs aggregates inside hela cells to achieve ultra longterm cellular. Nanoparticles combining enhanced cellular drug delivery with efficient fluorescence detection are important tools for the development of theranostic agents. Aggregationinduced emission aie, which was first introduced by tang et al. Photoactivatable aggregationinduced emission probes for lipid. The discovery of the aie effect opens a new avenue for the development. A fluorescent sensor based on aggregationinduced emission.
Conversion of the fluorescent properties depending on the aggregated states can also provide information on the evolution of. The polymer exhibits unique aggregationinduced emission aie characteristics and can selfassemble to sizetunable particles in dmfwater mixtures. Photostable fluorescent organic dots with aggregation. Aggregationinduced emission aie is an abnormal phenomenon that is observed with certain organic luminophores fluorescent dyes most organic compounds have planar structures and higher photoemission efficiencies in solution than in the solid state. Selfassembled gold nanoclusters for bright fluorescence.
Aggregationinduced emission aie active fluorescent probes with multiple binding sites toward atp sensing and live cell imaging hengchang ma, a manyi yang, a caili zhang, b yucheng ma, a yanfang qin, a ziqiang lei, a lu chang, a lei lei, a tao wang a and yuan yang a. Request pdf preparation of biocompatible aggregationinduced emission homopolymeric nanoparticles for cell imaging a series of new homopolymers. Recently, aggregationinduced emission aie fluorophores have emerged as attractive bioimaging tools due to flexible controllability, negligible toxicity and superior photostability. A phresponsive drug delivery system with an aggregation induced emission feature for cell imaging and intracellular drug delivery. Many efforts have been made to develop new photosensitizers for efficient cancer photodynamic therapy. Densil s1, chang ch2, chen cl2, mathavan a1, ramdass a3, sathish v4, thanasekaran p2, li ws2, rajagopal s5. Aggregationinduced emission enhancement of anthracenederived schiff base compounds and their application as a sensor for bovine serum albumin and optical cell imaging. Aggregationinduced emission luminogens for biomedical. Applications is the first book to explore the hightech applications of aie luminogens, including technological utilizations of aie materials in the areas of electroluminescence, mechanochromism, chiral recognition, ionic. In order to improve the cellimaging ability, and particularly, to extend the bioapplication of aiegen, human papillomavirus hpv capsid protein l1 was assembled with the complex of dna and aggregationinduced emission fluorogen 9,10distyrylhydrazine dsai, where the viruslike particles vlps of hpv encapsulate the complex via electrostatic interaction. Aggregation induced emission enhancement and living cell imaging of novel diarylanthracene conjugated dyes c. The luminogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution.
Live cell imaging with luminicell tracker nanoparticles. In this work, we report a novel nano photosensitizer, polymeric micelles aiem with aggregation induced emission. Photo and aromatic stackinginduced green emissive peptidyl nanoparticles for cell imaging and monitoring of nucleic acid delivery. Authors contributing to rsc publications journal articles, books or book chapters do. Recent years have witnessed the fast growth of fluorogens with aggregationinduced emission characteristics aiegens in biomedical research. Photobleaching resistance of this synthesized nanoparticle is tested and compared with its similar counterpart, which proves its superiority and capability of long term uorescence emission.
Aptamerdecorated selfassembled aggregationinduced emission. We utilize the objectivebased total internal reection microscopy combined with the living cell incubation. Methods in molecular biology methods and protocols, vol 991. Preparation and selfassembly of amphiphilic polymer with. Aie is a novel photophysical phenomenon, which offers a new platform for researchers to look into the lightemitting processes from luminogen aggregates, from which useful information on structureproperty. Here we show the entire gelation process of chitosan liohurea aqueous system by aggregationinduced emission fluorescent imaging. Moreover, the acq dyes with highly planar conjugated structure also exhibit small stokes shift and suffer from reabsorption of the dye emission, further reducing their emission efficiency 8. Xiaomei liu and gaolin liang cas key laboratory of soft matter chemistry, department of chemistry, university of science and technology of china, hefei, anhui 230026, china hefei corresponding author.
An amphiphilic polymer bearing tetraphenylethene tpe moiety was synthesized by convenient reactions. Aggregationinduced emission probes for cancer theranostics. Photostable fluorescent organic dots with aggregationinduced. Photoactivatable aggregationinduced emission probes for lipid dropletsspecific live cell imaging. Addition of poor solvents induces the molecules to aggregate, which turns the emission on and boosts their luminescence efficiencies dramatically aggregationinduced emission or aie. Applications is the first book to explore the hightech applications of aie luminogens, including technological utilizations of aie materials in the areas of electroluminescence, mechanochromism, chiral recognition, ionic sensing, biomolecule detection, and cell imaging.
Aggregationinduced emission luminogen with nearinfrared. Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregationinduced emission aie properties, which can be used for longterm cell tracking. Aggregation induced emission active cytophilic fluorescent bioprobes for longterm cell tracking. Aggregation induced emission of fluorescent bioprobes and methods of using the same. Hong fluorescent probes, cell imaging, protein misfolding. A novel aggregationinduced emission fluorescent probe for. The weak emission of aiegens as molecular species and their bright luminescence as nanoscopic aggregates distinguish. Aptamerdecorated selfassembled aggregation induced emission organic dots for cancer cell targeting and imaging.
Aggregationinduced emission enhancement and cell imaging of a. Luminicell live cell tracking nanoparticles sigmaaldrich. The aggregation induced emission aie feature offered silole biolabels the sensitivity 40 to 140fold higher than that of a startoftheart immunoassay using directly fluorescentlabeled. Preparation of biocompatible aggregationinduced emission. Aggregationinduced emission aie polymeric micelles for. Photostable fluorescent organic dots with aggregationinduced emission aie dots for. Aggregationinduced emission applications ebook epub. Dual aggregation induced emission for enhanced fluorescence sensing of furin activity in vitro and in living cells. Aggregationinduced emission by ben zhong tang overdrive. Ultrabright organic dots with aggregationinduced emission. Fluorescent dyes with aggregationinduced emission provide.
Aggregationinduced emission for highly selective and sensitive fluorescent biosensing and cell imaging liang huang, liming dai department of macromolecular science and engineering, center of advanced science and engineering for carbon case4carbon, case western reserve university, 10900 euclid avenue, cleveland, ohio 44106. Applications is the first book to explore the hightech. This book explores the aggregationinduced emission aie effect, which has opened new avenues for the development of advanced luminogenic materials in the aggregate or solid state. The fluorescent molecules can readily pass through cell membranes, stain only the. Fullrange intracellular ph sensing by an aggregation. Article in small 1247 september 2016 with 299 reads how we measure reads. On the one hand, novel bioprobes with superior physicochemical. Dual aggregationinduced emission for enhanced fluorescence.
A phresponsive drug delivery system with an aggregationinduced emission feature for cell imaging and intracellular drug delivery. Jun 23, 2016 here we show the entire gelation process of chitosan liohurea aqueous system by aggregation induced emission fluorescent imaging. All of these dha derivatives displayed distinct aggregationinduced emission aie. Activatable cellpenetrating peptide conjugated polymeric nanoparticles with gdchelation and aggregationinduced emission for bimodal. Aggregationinduced emission aie is a novel photophysical. A novel red aggregationinduced emission enhancement aiee chromophore named bcpabodipy was synthesized based on the construction units 4n. A fluorescent bioprobe comprising one or more fluorogens labeled on biomolecules. Aggregation induced emission luminogens aiegens for nondoped organic lightemitting diodes han nie, jian huang, zujin zhao, and ben zhong tang chapter 7, pp 173198. The switchable emission manipulated by hydrogen bonding provided solid evidence for the restriction of intramol. Aggregationinduced emission on supramolecular coordination. Aggregationinduced emission aie molecules eliminate the.
Supramolecular hyaluronic assembly with aggregation. By enabling light emission in the practically useful solid state, aie has the potential to significantly expand the technological applications of luminescent materials. Jan 28, 20 activatable cell penetrating peptide conjugated polymeric nanoparticles with gdchelation and aggregation induced emission for bimodal mr and fluorescence imaging of tumors bin xia, xu yan. The emergence of the aggregationinduced emission aie concept. A novel red aggregationinduced emission enhancement aiee chromophore named bcpa bodipy was synthesized based on the construction units 4n,nbis43,6ditertbutylcarbazol9yl phenyl aminobenzaldehyde bcpa and fluorogen. Luminicell is founded based on the novel technology of aie, which takes the advantage of. Otherwise said, these fluorophores or fluorescent dyes are much more emissive in solution compared to just the solid form, in that the. Fluorescence imaging and multiple therapeutic functions of aggregationinduced emission aiebased cancer theranostics. Applications is the first book to explore the hightech applicationsof aie luminogens, including technological utilizations of aie materials in the areas of electroluminescence, mechanochromism, chiral recognition, ionic sensing, biomolecule detection, and cell imaging. Supramolecular aggregationinduced emission aie has become a research hotspot in cell imaging.
Cationic quaternized chitosan bioconjugates with aggregation. Fluorescence imageguided chemotherapy the integration of diagnostic imaging and therapeutic modalities in one molecule provides a simple and efficient approach for monitoring drug distributions and evaluating therapeutic. Herein, we recapitulate the current advances in the application of aie fluorophores to monitor nddss both in vitroand in vivo. In this chapter, we introduce the marriage between aggregation induced emission and supramolecular coordination complexes, in which the two concepts melt into one that enables a new kind of lightemitting metalorganic materials based on discrete metallacycles and metallacages.
Fluorescence imaging and multiple therapeutic functions of aggregation induced emission aiebased cancer theranostics. Edited by professor tang, who first discovered this phenomenon, this 2volume reference addresses the fundamentals of aggregationinduced emission aie. Herein, supramolecular assembly with aie effect was constructed in two stages, where adamantane modified tetraphenylethene selfassembly emitted weak fluorescence, and then after adding. Coassembly of hpv capsid proteins and aggregationinduced. Aggregationinduced emissionfluorophores and applications. A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging bin li, a tianhong chen, b zhipeng wang, d zhiming guo. Recent progress of bodipy dyes with aggregationinduced. Aggregationinduced emission compounds based on 9,10. Aggregationinduced emission ebook por ben zhong tang. Oct 25, 2019 synthesis, solvatochromism, aggregationinduced emission and cell imaging of tetraphenylethenecontaining bodipy derivatives with large stokes shifts. Due to their high biocapacity and fluorescence, they can help researchers to find and mark the location of proteins. Since the light emission was induced by aggregate formation, professor tang termed the process aggregationinduced emission aie.
Fabrication of fluorescent silica nanoparticles with. Accompanied by other pseudo in situ investigations, we propose. Fullrange intracellular ph sensing by an aggregationinduced emissionactive twochannel ratiometric fluorogen sijie chen, yuning hong, yang liu, jianzhao liu, chris w. The polymer nanoparticles can be used for cell imaging, which provides a potential stable fluorescent tool to monitor the distribution of drugs and bioconjugates in living cells.
Lightenhanced bacterial killing and less toxic cell imaging. In addition, our recent discovery that aiegens with high brightness and efficient reactive oxygen species generation in the aggregate state further expand their applications in imageguided cancer surgery and therapy. Aggregationinduced emission enhancement and living cell. Principles and applications of aggregationinduced emission. Restriction of intramolecular motion is the working mechanism of aggregation. Recent progress of bodipy dyes with aggregationinduced emission.
Aggregationinduced emission aie fluorophores as imaging. The polymer nanoparticles can be used for cell imaging, which provides a potential stable fluorescent tool to monitor the distribution of drugs. Organic fluorogens with aggregationinduced emission aie characteristics have demonstrated their potential to be ideal candidates for live cell imaging. Aggregationinduced emission enhancement of anthracene.
A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging. The aggregationinduced emission aie is the phenomena that the fluorescence intensity is low in solution, the aggregated state shows strong emission. Gelation process visualized by aggregationinduced emission. Currently, a serious problem obstructing the largescale clinical applications of fluorescence technique is the shallow penetration depth. A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging bin li, a tianhong chen, b zhipeng wang, d zhiming guo, d jhair pena, a lintao zeng bc and jinfeng xing a. Yuan y, xu s, cheng x, cai x, liu b 2016 bioorthogonal turnon probe based on aggregationinduced emission characteristics for cancer cell imaging and ablation. Aggregationinduced emission by tang, ben zhong ebook. Aggregationinduced emission aie is a novel photophysical phenomenon which offers a new platform applications for researchers to look into the lightemitting processes from luminogen aggregates, from which useful information on structureproperty relationships may be collected and mechanistic insights may be gained. Photostable aie luminogens for specific mitochondrial imaging and its method of manufacturing thereof. Imaging intracellular anticancer drug delivery by self.
The photosensitizer is a necessary component of photodynamic nanomedicine. Alternative cell tracking methods are therefore desired to attain reliable, stable, and efficient labeling over a long time. Aggregationinduced emission ebook by ben zhong tang. Induced emission homopolymeric nanoparticles for cell imaging chunping ma pcfm lab, dsapm lab, klghei of environment and energy chemistry, state key laboratory of optoelectronic materials and technologies, school of chemistry and chemical engineering, sun yat.
Sean lim chief editor the skeptical chemist linkedin. Fluorescent dyes with aggregationinduced emission provide new probes for cancer diagnosis and therapy. Photodynamic therapy pdt is a noninvasive treatment for selectively killing malignant tumor cells. Opposite to conventional organic dyes, the aie luminogens are nonluminescent when molecularly dissolved but highly emissive upon aggregation. The book presents an overview of this rapidly emerging and exciting area of research, inviting scientists to renew their photophysical knowledge and stimulate new developments in the field. Twophoton fluorescence microscopic imaging with excitation in the longerwavelength nearinfrared nir region 1100 nm and emission in the niri region 650950 nm is a good choice to realize deeptissue and highresolution imaging. The photoluminescence pl of t 2 tps and tpe layers adsorbed on the tlc plates can be turned off and on continuously and reversibly by solvent exposure. Aggregationinduced emission enhancement and cell imaging. A novel crosslinked nanoparticle with aggregationinduced. Synthesis, solvatochromism, aggregationinduced emission and cell imaging of tetraphenylethenecontaining bodipy derivatives with large stokes shifts.
Fluorescence emission polymer is a kind of polymer which can absorb light of certain frequency and then give out light. This is the first time that erythrocytes have been visualized in the brain through a fluorescence. A novel aggregationinduced emission fluorescent probe for nucleic acid detection and its applications in cell imaging author links open overlay panel xiaowei xu shengyong yan yimin zhou rong huang yuqi chen jiaqi wang xiaocheng weng xiang zhou. Applications is the first book to explore the high tech. In this work, we have successfully developed ultrabright organic dots with aggregationinduced emission aie dots and demonstrated their capabilities for cellular imaging and cell tracking.
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